Übersetzung für "Recombinantly expressed" in Deutsch

In particular the antigens can be cloned in bacterial expression vectors and expressed recombinantly.

Various biophysical methods require large amounts of recombinantly expressed and purified proteins.

To this end, cDNA expression libraries were prepared from fresh tumor tissue and recombinantly expressed as proteins in suitable systems.

The nucleic acid sequences to be expressed recombinantly may be present in the gene construct as one or more copies.

One or more copies of the nucleic acid sequences to be expressed recombinantly may be present in the gene construct.

In order to confirm these results from this yeast test system, recombinantly expressed 163 [expressed together with glutathione transferase (GST) as a fusion protein] was bound to a column and an attempt was then made to purify 35 S-labeled p27 protein by means of affinity chromatography on this column.

In general, however, it is expedient to carry out all process steps for the isolation of the protease and of the corresponding proenzyme from a solution containing these proteins, such as plasma, plasma fractions, tissue fluids or cell culture supernatants of the recombinantly or transgenically expressed protease or mutants thereof in the presence of protein stabilizers.

Prokaryotic cells, in particular E. coli cells, are unproblematic to handle but have a number of disadvantages when eukaryotic proteins are expressed recombinantly.

The following peptides or a purified protein which was recombinantly expressed in E. coli were used for propagating these antibodies:

The following peptides or purified protein which was recombinantly expressed in E. coli were used for propagating these antibodies:

Proteins and polypeptides described according to the present technology may be isolated from biological samples such as tissue or cell homogenates and may also be expressed recombinantly in a multiplicity of pro- or eukaryotic expression systems.

Medigene also analyzes if the TCRs leads can be recombinantly expressed in primary human T cells by tests for function and specificity.

Following the mutagenesis, the protein which is encoded can be expressed recombinantly, and the activity of the protein can be determined, for example using the tests described in the present text.

Fusion proteins can, for example, be separated more easily from other proteins or can be recombinantly expressed in larger amounts.

Alternatively, the GPIba protein used may be fused at the N terminus to a heterologous signal sequence, i.e., to a polypeptide which normally is not present in the human GPIba polypeptide but which has a beneficial influence on expression and/or secretion of the recombinantly expressed GPIba protein in the chosen expression system.

The disclosure includes a method for the production of the protein according to the disclosure wherein this is recombinantly expressed and purified.

Cleavage sites for proteases which are suitable as tags for recombinantly expressed proteins are well known to a person skilled in the art.

Alternatively, the GPIba protein used can, at the N-terminus, be fused to a heterologous signal sequence, i.e., to a polypeptide not usually present in the human GPIba polypeptide, which signal sequence, however, positively influences the expression and/or secretion of the recombinantly expressed GPIba protein in the selected expression system.