Übersetzung für "Basal medium" in Deutsch

Fibroblast cells originating from juvenile, mesenchymal human tissue were cultured in basal medium according to "Eagle" of the company Bohringer, Mannheim, Federal Republic of Germany, to which additionally bovine serum albumin had been added.

The cells used were monkey kidney cells of the strain RC-37 Rita (Italodiagnostics, Rome), which were cultured in basal Eagle's medium (BME; Eagle, H., J. Exp.

For example, fibroblasts may be isolated from juvenile, mesenchymal tissue and slightly be reproduced by cell division in a basal medium according to "Eagle" of the company Bohringer, Mannheim, with an addition of bovine serum albumin.

HeLa cells were seeded in Linbro plates (10 5) and, after 6 hours, incubated at varying concentrations of cisplatin and a compound according to Example 1, respectively, in basal medium according to Eagle, which contained either 2.2 g (pH 7.4) or 0.85 g (pH 6.8) sodium bicarbonate and 10% fetal calf serum, at 37° C. in a 5% CO 2 atmosphere for 24 hours.

A mixture of Eagle's basal medium and sheep fetus serum (9+1) was used as the cell culture medium for all investigated cell lines.

The cells used were monkey kidney cells of the strain RC-37 Rita (Italodiagnostics, Rome), which were cultured in basal Eagle's medium (BME;

The HUVECs are cultivated at 37° C. and 5% CO 2 in basal medium (PromoCell, C-22200) with supplement mix (PromoCell, C-39225).

After differentiation of 3T3-L1 fibroblasts to form adipocytes, 65000 cells per well are seeded in a 96 well plate and incubated at 37° C. for four hours with basal medium (DMEM+10% fetal bovine serum) and then incubated for an hour with serum-free medium.

Cultivation of the cells was carried out in basal medium (10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 ?g/ml streptomycin;

The test substances dissolved in DMSO were used to prepare in each case one dilution series in Vasculife VEGF low-serum medium [basal medium with LifeFactors (Cellsystems, LM-0002), without FCS, with 0.1% BSA] without sodium orthovanadate and one dilution series in Vasculife VEGF low-serum-medium with 8 mM sodium orthovanadate in the desired concentrations in the range from 10 ?M to 10 ?M, the final DMSO concentration being 1%.

3T3-L1 fibroblasts are cultivated at 37° C. and a 5% CO 2 content with Dulbecco's Modified Eagle Medium (DMEM) having 10% FBS, 4% L-Glutamin and 1% Penicillin/Streptomycin (=basal medium).

After having spent between three and five days in basal medium, the adipocytes are optimally differentiated and show by inclusion of lipid droplets the phenotype that is typical for adipocytes.

Media that have been used for cultivating tumor cells such as RPMI 1640, Basal Medium Eagle, ISCOVE's, Medium 199, Leibovitz L-15, etc. are incapable of appropriately enhancing cancer cell growth while preventing the growth of normal cells and bacterial contaminants.

After one to three weeks in each case, the explants are transferred to fresh medium until plumules and small shoots develop, which are then transferred to the same basal medium including timentin and PPT or alternative components with growth regulators, namely, for example, 0.5 mg/l indolylbutyric acid (IBA) and 0.5 mg/l gibberillic acid GA 3, for rooting.

After in each case from one to three weeks, the explants are transferred to fresh medium until plumules and small buds develop which are then transferred to the same basal medium including Timentin and PPT or alternative components comprising growth regulators, that is to say, for example, 0.5 mg/l of indolylbutyric acid (IBA) and 0.5 mg/l of gibberillic acid GA 3, for rooting.

Strain HCM-10 was concentrated from ground water on a basal medium (table 1) containing tert-butyl alcohol as sole carbon and energy source.

After in each case from one to three weeks, the explants are transferred to fresh medium until plumules and small buds develop which are then transferred to the same basal medium including Timentin and PPT or alternative components with growth regulators, that is to say, for example, 0.5 mg/l indolylbutyric acid (IBA) and 0.5 mg/l of gibberillic acid GA 3, for rooting.

After one to three weeks in each case, the explants are transferred to fresh medium until plumules and small shoots develop, which are then transferred to the same basal medium including timentin and PPT or alternative components with growth regulators, namely, for example, 0.5 mg/l indolylbutyric acid (IBA) and 0.5 mg/l gibberellic acid GA3, for rooting.

The method of any one of claims 1-19, wherein the cell culture is a fed-batch culture and wherein the cell culture enhancing reagent(s) is/are present in the basal cell culture medium and/or in the feed medium.