Übersetzung für "Cell disruption" in Deutsch

The cell disruption necessary for isolation of the DATA is effected by an enzymatic route.

The cell disruption necessary to isolate the DATA is carried out by enzymatic means.

The disruption of the cells is carried out using the methods normally used for cell disruption.

The cell disruption serves the purpose of exposing them to increased disintegration.

It is found that the compounds of the invention protect HIV-infected cells against virus-induced cell disruption.

The disruption of the cells after irradiation with UV light is carried out using methods normally used for cell disruption.

Especially preferred in process step B) is the disruption by hot water in combination with an enzymatic cell disruption.

For this purpose the PQQ can be added during cell culturing, during cell disruption or prior to purification.

Cell disruption in small and large volumes (mixer mill, ultra-sonic disruption, bead ruptor, high pressure homogenizer)

Following cell disruption, DNA can easily be isolated through precipitation with acid or alcohol (NaOAc /ethanol).

Thinner gauge needles can cause cell disruption during injection, and may compromise cell viability and therefore may affect efficacy of treatment.

Eribulin exerts its effects via a tubulin-based antimitotic mechanism leading to G2/M cell-cycle block, disruption of mitotic spindles, and, ultimately, apoptotic cell death after prolonged and irreversible mitotic blockage.

Methods which are usable for cell disruption include repeated freezing and thawing, mechanical disruption, e.g. homogenizing with a motor-driven TEFLON (polymerized tetrafluoroethylene) pestle in a glass homogenizer, bursting by osmotic shock, disruption by means of ultrasonic vibration and the use of lytic chemical agents such as anionic detergents, for example sodium dodecyl sulphate (SDS), lithium dodecyl sulphate, sodium 4-aminosalicylate, sodium dodecyl sarcosinate or sodium tri-isopropylnaphthalene sulphonate.

As an alternative, the aldolase can be separated, particularly expediently after cell harvesting and disruption, from biomass ballast by aqueous 2-phase extraction.

It is therefore an object of the invention to provide a process for reducing the salt concentration in a biomass-containing suspension, in particular in the salt-containing lower phase of an aqueous two-phase extraction of intercellular proteins after cell disruption, which can be used commercially without difficulties.

For the process according to the invention, precursors of insulins or of insulin derivatives of the formula II can be employed that are still contaminated with a large number of proteins which originate from the fermentation solution and from the microorganisms directly after the cell disruption.

The subsequent cell disruption can be carried out, for example, as selected by means of ultrasound or Ultraturrax apparatuses, homogenizers or by freezing.

After cell disruption, the cytosolic fraction can be used without further purification, by means of which the expenditure of work is minimized.

After cell disruption, the insoluble components (inclusion bodies) are removed in any suitable manner, preferably by centrifugation or filtration following one or more washing steps with agents that leave IBs intact but possibly dissolve foreign cellular proteins, e.g. in water or phosphate buffer, optionally with mild detergents added such as Brij®.

For the process according to the invention, proteins of the formula II can be employed which, directly after cell disruption, are still contaminated with a multiplicity of proteins derived from the fermentation solution and from the microorganisms.

The culture is then shaken further in a shaker in a waterbath at 40° C. The mutarotase activity is detected after the subsequent cell disruption.

Other methods for disrupting the cell membranes of the biological material are thermal cell disruption in which the cell membranes are denatured by the effects of temperature or the combination of thermal and mechanical methods.

Constant Systems Distributes Disruptors We have now processed 9 machine orders/sales to Canada in the last 3 years and we are gaining sales from the local competition by steadily building a solid reputation for Cell Disruption equipment in Canada.