Translation of "Dna purification" in German

Inhibitors from DNA purification (i.e. SDS)

The DNA purification in this case is based on the wellestablished Spin Filter technology.

This Kit offers a timesaving, easy and high yield DNA purification system.

In addition, DNA purification from the authentic sample cannot be performed by this method.

Having digested the isolated vector DNA (Qiagen Plasmid Purification Kit) with the restriction enzymes MluI and NotI, the correspondingly digested 96 different PCR bands described in Example 5d are ligated in, the resulting ligation products are transfected in E. coli and then a highly complex mixture (>10 6 different transformants) is isolated from vector DNA (pBS FRTclone-vlambda) (Qiagen Plasmid Purification Kit).

The multistage cloning process, generally taking place via microorganisms such as E. coli (e.g. DNA isolation, purification, analysis, recombination, cloning in bacteria, isolation, analysis etc.), is therefore reduced to the last transfer of the DNA molecule into the final effector cells.

All DNA enrichment/purification methods are suitable, including salt precipitation, phenol/chloroform extraction, columns and batch methods.

This difference in size between the fetal and the maternal nucleic acids could be exploited in order to be able to enrich and therefore investigate in more detail fetal DNA, if suitable purification methods were available.

The entire batch was then purified with the DNA Purification Kit (list of chemicals) before the further transformation.

The restriction hydrolysates were purified by phenol/chloroform extraction and precipitated in salt and ethanol or by DNA purification (Qiagen).

The multi-stage cloning process which is mostly carried out in microorganisms such as E. coli (e.g. DNA isolation, purification, analysis, recombination, cloning in bacteria, isolation, analysis, etc.) is thus reduced to the last transfer of the DNA molecule into the final effector cells.

The absence of added chaotropic substances leads to substantial improvements and purifications in the subsequent DNA purification procedure and/or in the corresponding transfection of target cells, that is for cells of both eukaryotic and prokaryotic origin.

The restriction hydrolysates can be purified by phenol/chloroform extraction and precipitated in salt and ethanol or by DNA purification (Qiagen).

For example, DNA extraction with subsequent DNA purification and subsequent mixing with components for DNA amplification and subsequent aliquoting into many small droplets and subsequent digital DNA-amplification in the aliquots can be realized with minimum handling effort and low contamination risk by using the droplet generation described herein.

In that way, droplet generation can be connected to other fluidic operations via channels as well as to an inlet, which allows, for example, automated DNA extraction, DNA purification, DNA preamplification and subsequently digital PCR (and digital isothermal verification methods, respectively).

The steps that follow lysis (binding and washing the DNA; final elution) guarantee highly efficient DNA purification.

The Ron's Plasmid Mini Kit presents remarkable features of timesaving, easy, prompt and low cost plasmid DNA purification from E. coli and other bacteria.