Translation of "Proteolytic degradation" in German

The biological activity of the various expected proteolytic degradation products has not been assessed.

The proteolytic degradation was carried out with various proteases.

Thus, the purification did not lead to a proteolytic degradation of high-molecular vWF multimers in the complex.

These triplet structures are created by proteolytic degradation of vWF multimers and indicate an instability of the vWF multimers.

Factor VIII is stabilized by complex formation with vWF and protected from proteolytic degradation thereby.

The androgen receptor binds androgens, by which it is stabilized and protected from a quick proteolytic degradation.

The alpha chain in particular is affected by incipient proteolytic degradation by fibrinogen-degrading proteases.

As a result of proteolytic degradation, a rapid loss of biological activity can also be observed.

In this way, the last proline residue should protect the resulting protein from proteolytic degradation during production and purification.

They shield peptides against proteolytic degradation and can be functionalized for cell type- or organ-specific targeting.

Nonclinical studies have shown that exenatide is predominantly eliminated by glomerular filtration with subsequent proteolytic degradation.

Vestronidase alfa is a recombinant human enzyme and is therefore eliminated by proteolytic degradation into small peptides and amino acids.

It has now been found, surprisingly, that by means of novel intermolecular and/or intramolecular crosslinking of the macromolecules of the intercellular matrix of organs or parts of organs, prior to or even without the proteolytic degradation by means of ficin, the biophysical characteristics originally present, such as, for example, in the case of vascular prostheses, the axial and radial elasticity and the smooth nature of the inner surface of the vessels, are substantially retained.

A low contrast (as in the marker lane of the image) between bands within a lane indicates either the presence of many proteins (low purity) or, if using purified proteins and a low contrast occurs only below one band, it indicates a proteolytic degradation of the protein, which first causes degradation bands, and after further degradation produces a homogeneous color ("smear") below a band.

In addition, the inadequately hydroxylated collagen cannot be incorporated in the collagen matrix, and it very readily undergoes proteolytic degradation.

Modifications of the preparation make it possible to liberate the HS I polysaccharide or peptido polysaccharide by alkali degradation (e.g. incubation into 0.5M NaOH for 12 h at 4° C.) or proteolytic degradation (e.g. with papain or pronase) or other methods during each single step of the preparation, also from untreated cells or from the untreated medium, and it can be purified by gel chromatography, ion exchange chromatography, density gradient ultracentrifugation, HPLC on gel permeation columns, on silica gels and/or reversed phase columns.

In particular, they are stable against a proteolytic degradation, they are autoclavable, and can also be used in an acid and alkaline medium (pH 1 to 13), as well as together with highly concentrated chaotropic agents (5M guanidine hydrochloride, 8M urea).

P-membrane layers of this type can finally also find application as capsule-envelopes for pharmaceutical preparations administered orally, whereby the desired release of the content is caused only by the proteolytic degradation in specific sections of the digestive tract.

The underhydroxylated collagen is additionally unable to be incorporated in the collagen matrix and very easily undergoes proteolytic degradation.

Krausslich and his collaborators also showed that although the presence of an active 2A is absolutely necessary for the proteolytic degradation of p220 during the host cell shutoff, this degradation is not directly carried out by 2A (Krausslich, H. G. et al., loc.

Possibly, the two proteinases 2A and 3C are directly or indirectly involved in the proteolytic degradation of another 14 cellular proteins during the infection of HeLa cells with poliovirus (Urzainqui, A. and Carrasco, L., J. Virol.

P-membrane layers of this type can finally also find application as capsule-envelopes for pharmaceutical preparations administered orally, whereby the desired release of the content is caused only by the proteolytic degradation in specific sections of the digetive tract.

Since as a result of the high proteolytic degradation rate (in-vivo half-life) of the calcitonins therapeutically employed, at present comparatively high amounts have to be administered, this—as described above—in turn leading to rapid antibody formation (secondary resistance) and possible side effects (contraindications), the increase in the bioactivity or proteolysis resistance is ascribed great importance.

In contrast to the 20-membered ring structure known from the prior art, the calcitonins according to the invention are more stabilized with respect to their conformation, such that proteolytic degradation is retarded.

Such an inactivation of factor VIII may, e.g. be effected by dissociation by using e.g. a chelating agent, by degradation, in particular proteolytic degradation, e.g. by means of serine proteases, such as thrombin or activated protein C, by the binding of affinity partners, such as antibodies or peptides.

The recombinant vWF multimer molecules exhibit a high structural integrity and do not contain any proteolytic degradation products, as compared to the vWF triplet structures of plasmatic vWF known from the literature.

According to the invention, this object is achieved by providing a factor VIII/vWF-complex which particularly contains high-molecular vWF multimers and which is free from low-molecular vWF molecules and from proteolytic vWF degradation products.