Translation of "Colorimetric assay" in German

The method is based on a competitive colorimetric ELISA assay.

The Bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration.

Description: The Bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration.

This is a colorimetric dye-binding assay based on differential color change in response to various concentrations of protein.

Label-free gold nanoparticles are used in a selective colorimetric assay (see picture) and in a highly sensitive DLS assay for the recognition of arsenic in concentrations as low as 3 ppt.

The reporter genes used in the 2-hybrid system are enzymes which can be used to detect said protein interaction either by growth selection or by a simple colorimetric assay and which are very sensitive.

Proliferation can be monitored by methods known in the art, such as measurement of metabolic activity, for example with standard XTT colorimetric assay (Boehringer Mannheim) measured by standard ELISA plate reader at OD 490/560, by measuring 3 H-thymidine incorporation into DNA following an 8 h culture with 1 pCu 3H-thymidine, harvesting the cells onto glass fibre mats using a cell harvester and measuring 3 H-thymidine incorporation by liquid scintillation counting, or by staining techniques, such as Crystal Violet staining.

The “Reverse Transcriptase Assay, colorimetric” (Roche Diagnostics GmbH, Mannheim, Germany) is used in accordance with the manufacturer's information.

The Mosmann colorimetric assay (Mosmann 1983) involves the conversion of the colourless MTT salt (dimethylthiazole diphenylterazolium bromide) to blue, water-insoluble formazan crystals by mitochondrial dehydrogenases.

Alternatively, the dihydroorotase may also be measured in a less sensitive colorimetric assay by the method of Prescott and Jones (Anal.

The existence of endotoxin can be measured by four different methods (gel-clot, turbidimetric, colorimetric and chromogenic assay).

Proliferation can be monitored by methods known in the art, such as measurement of metabolic activity, for example with standard XTT colorimetric assay (Boehringer Mannheim) measured by standard ELISA plate reader at OD 490/560, by measuring 3 H-thymidine incorporation into DNA following an 8 h culture with 1 ?Cu 3 H-thymidine, harvesting the cells onto glass fibre mats using a cell harvester and measuring 3 H-thymidine incorporation by liquid scintillation counting, or by staining techniques, such as Crystal Violet staining.

The detection of the proliferation activity was made by means of a semi-quantitative MTT-based colorimetric assay (EZ4U, Biozol).

On the other hand, the usual colorimetric assay tends to overestimate plasma creatinine concentration, so both effects tend to cancel each other out.

Supplier: VWR Chemicals Description: The Bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration.

The detection of the proteins bound to phages or phage proteins can be performed by using a colorimetric assay, detecting, e.g., NADH (Bergmeyer & Bern;

Protein interactions can be detected indirectly in these systems after transcomplementation of the abovementioned enzymes by way of growth selection or of fluorimetric or colorimetric enzyme detection assays.

The colorimetric assays are generally usable to detect the activity of intracellular membrane or periplasmatic enzymes, or of cell components or products of phage reproduction, e.g., phage proteins or phage nucleic acids.

The colorimetric assays may be identical for all bacteria to be analyzed, but they may also be specific for particular combinations of bacteria/phages.

For the colorimetric test assays, chemiluminescence-, or bioluminescence, absorption, fluorescence or circular dichroism detection methods are employed, depending on the sensitivity desired.

The most common applications are the concentration determination of nucleic acids – DNA and RNA – the purity of nucleic acids, the concentration determination of proteins by direct measurement or colorimetric assays, the study of enzymatic reactions, and monitoring growth curves of bacterial cell suspensions.