Übersetzung für "Cell sorter" in Deutsch

This is preferably carried out by means of a fluorescence-activated cell sorter.

The cell sorter delivers the separated cells via feed lines to the applicator.

As a postdoc at the GBF he established the high-speed cell sorter MoFlo.

In a final stage, an industrial solar cell tester and sorter records the electrical parameters of the experimental solar cells.

3 to 4 weeks after fusion, positive primary cultures are cloned with the help of a fluorescence-activated cell sorter.

In the cell sorter, the signals of single droplets are measured in which single cells may be present.

The Herzenberg Laboratory at Stanford has integrated JMP with the Fluorescence Activated Cell Sorter (FACS).

The hybrid cells hereby formed are cloned in the usual way, for example with the use of a commercially available cell sorter, and the clones obtained, which form the desired monoclonal antibodies, are cultured.

Primary cultures which showed the desired cross-reaction were cloned using FACS (fluorescence activated cell sorter) in 96-well cell culture plates.

Positive primary cultures (see Example 3) are, 3 to 4 weeks after fusion, cloned with the help of a fluorescent-activated cell sorter.

Primary cultures which showed no or only small cross-reaction with HbA0 were further cloned with the help of a fluorescent-activating cell sorter (FACS) on 96 well cell culture plates (form Nunc).

Positive primary cultures (see Example 3) are, 3 to 4 weeks after fusion, cloned with the help of a fluorescence-activated cell sorter.

The hybrid cells hereby formed are cloned in conventional manner, for example with the use of a conventional cell sorter, and the clones obtained, which form the desired monoclonal antibodies, are cultured.

On the level of a cell, this means virtually that the method according to the invention is able to distinguish between different molecules bearing a chromophore and being present in the measuring volume whereas in the cell sorter, the concentration of this chromophore is determined integrally in the droplet, irrespective of whether it is part of a small molecule or it occurs in a complex or bound to a cell.

The primary cultures of hybrid cells that are formed in this process are cloned in the usual manner for example by using a commercial cell sorter or by "limited dilution" and cultures which react positively towards an amphetamine in a suitable test procedure are processed further.

Positive primary cultures are cloned two weeks after fusion using a fluorescence-active cell sorter (Beckton Dickinson).

The DNA content of the cells is determined in a fluorescence-activated cell sorter after staining with Hoechst 33258 (Lucibello et al., EMBO J. 132 (1995)).

The sorter cell can also have a conveyor belt, in particular a transverse belt, that preferably runs horizontally, transversely to the direction of travel of the driving unit and, in this way, it picks up the products to be sorted from a loading plane, for example of a high-reach rack, past which the driving unit travels, and, following their transport, sets the products again in a discharge plane, of the rack system, for example.

The novel compounds of the formula I, in which X is a fluorescent dye residue which is linked by way of a —CONH—, —COO—, —NH—C(?S)—NH—, —NH—C(?O)—NH—, —SO 2 NH— or —NHCO— bond, can be used as diagnostic markers in FACS (fluorescence activated cell sorter) analysis and fluorescence microscopy.

The primary cultures which contain the antigen-specific antibodies are cloned further using a fluorescence activated cell sorter on 96-well cell culture plates (Nunc Company).

Moreover, a magnetic process is known (MACS=magnetic activated cell sorter) which is obtainable commercially for separating large cell populations.

Primary cultures which exhibit a positive reaction with PSA-ACT and no cross-reaction with non-complexed PSA and non-complexed ACT or with all other serum components, are cloned in 96-well cell culture plates by means of a fluorescence activated cell sorter.

The present invention relates to a sorting apparatus having at least two sorter cells arranged substantially one above the other, in each case to pick up a product to be sorted, a horizontal loading plane assigned to the sorter cell and having a driving unit to which the sorter cells are fitted for moving the sorter cells from the respective loading plane to a horizontal discharge plane assigned to the sorter cell.

Before these cell species were employed for the adhesion experiments, their integrin expression was verified by means of fluorescence-labelled antibody against the alpha v beta 3 - or alpha v beta 5 -receptors and against the integrin subunits alpha v, beta 3 and beta 5 with the aid of a Becton-Dickenson fluorescence-activated cell sorter (FACS).