Übersetzung für "Cellular assay" in Deutsch

Other suitable cellular assay systems are known in the art.

The cellular assay for determination of the PDK1 kinase activity is performed as Luminex assay in the 96-well format.

The cellular assay for the determination of the PDK1 kinase activity is carried out as a Luminex assay in the 96-well format.

Following monthly intravitreal administration of Lucentis to patients with neovascular AMD, serum concentrations of ranibizumab were generally low, with maximum levels (Cmax) generally below the ranibizumab concentration necessary to inhibit the biological activity of VEGF by 50% (11-27 ng/ml, as assessed in an in vitro cellular proliferation assay).

Following monthly intravitreal administration of Lucentis to patients with neovascular AMD, serum concentrations of ranibizumab were generally low, with maximum levels (Cmax) generally below the ranibizumab concentration necessary to inhibit the biological activity of VEGF by 50% (11-27 ng/ ml, as assessed in an in vitro cellular proliferation assay).

The invention is based on the object of improving the sensitivity of the optical analysis of fluorescently labelled or luminescent cells in a cellular assay in order to be able to measure, for example, membrane potential changes which are as low as possible on the basis of fluorescence changes of potential-sensitive dyes.

The cellular assay for determination of the P-S473-PKB level is carried out as a Luminex assay in 96-well format in PC3 cells.

Inhibition of AKR1C3 of the inventive compounds in a cellular assay (the values reported are for at least two experimental determinations) Cellular

In a preferred embodiment the compounds disclosed in this invention do not posses any agonistic activity in a cellular assay up to a concentration of at least 7100 nM as shown in Example 32.

In this case, the biological test system may be configured as a cellular assay (adherent or suspension cells, primarily mammalian cells, but also plant cells, bacteria, fungi, yeasts or viruses) or else may comprise subcellular constituents, such as e.g. isolated cell nuclei or cytoplasm agglomerates, or else may comprise artificial carriers, such as e.g. plastic beads or glass microspheres, on which biologically active material, generally cellular or subcellular constituents, has been applied, an optical signal in the form of photons being liberated as a result of the interaction of different components.

The gene of the Photinus pyralis luciferase is moreover frequently used as reporter gene in cellular assay systems (Gould et al., Anal.

As a difference from the stably transfected PPARalpha reporter cell line, in the cellular PPARgamma assay the two components (luciferase reporter plasmid and PPARgamma expression plasmid) are transiently transfected into HEK cells because stable and permanent expression of the PPARgamma fusion protein is cytotoxic.

Besides the luciferase reporter plasmid pGL3basic-5×GAL4-TK and the PPARgamma expression plasmid pcDNA3-GAL4-humanPPARgammaLBD, also used for the cellular PPARgamma assay are the reference plasmid PRL-CMV (from Promega) and the plasmid pBluescript SK(+) from Stratagene.

In collaboration with researchers at Helmholtz Zentrum MÃ1?4nchen, biochemical and cellular assay systems shall be developed to search for new chemical molecules that influence specific biological processes.

Option 2 calculates the freely dissolved concentration/fraction in a selection of cellular assays.

HC cellular assays for phenotypes associated with neurodegeneration.

We use molecular and cellular assays to gain insight into disease mechanisms.

The primary metabolite exhibits similar potency compared to sunitinib in biochemical and cellular assays.

In a further application the method according to the invention may also be helpful in cellular reporting assays.

In cellular assays, ponatinib was able to overcome imatinib, dasatinib, and nilotinib resistance mediated by BCR-ABL kinase domain mutations.

In in vitro kinase and cellular assays, the metabolite, AP26123, inhibited ALK with approximately 3-fold lower potency than brigatinib.

Nonclinical in vitro experiments in nonsense mutation cellular assays and fish larvae cultured in an ataluren solution have shown that ataluren enabled ribosomal readthroughwith a bell-shaped (inverted-U shaped) concentration-response relationship.

Toceranib inhibited the activity of Flk1/KDR tyrosine kinase (vascular endothelial growth factor receptor, VEGFR2), platelet-derived growth factor receptor (PDGFR) and stem cell factor receptor (c-Kit) in both biochemical and cellular assays.

This inhibitory effect was confirmed in the ERK phosphorylation and cellular anti-proliferation assays in available melanoma cell lines expressing V600-mutant BRAF.

In cellular anti-proliferation assays the inhibitory concentration 50 (IC50) against V600 mutated cell lines (V600E, V600R, V600D and V600K mutated cell lines) ranged from 0.016 to 1.131 ?M whereas the IC50 against BRAF wild type cell lines were 12.06 and 14.32 ?M, respectively.

Nonclinical in vitro experiments in nonsense mutation cellular assays and fish larvae cultured in an ataluren solution have shown that ataluren enabled ribosomal readthroughwith a bell-shaped (inverted- U shaped) concentration-response relationship.

Toceranib inhibited the activity of Flk- 1/KDR tyrosine kinase (vascular endothelial growth factor receptor, VEGFR2), platelet-derived growth factor receptor (PDGFR) and stem cell factor receptor (c-Kit) in both biochemical and cellular assays.

Suitable methods include experimental non-clinical methods, such as, for example, in-vitro assays, in-vivo assays, cellular assays and animal models, and clinical methods or clinical studies, but are not restricted thereto.

The goal of the project is to combine a method comprising both cellular reporter assays and gene expression studies into a sensitive method.

CYTOO is a biotechnology company specialised in the development of physiologically relevant cellular models and assays for High Content Screening (HCS).

The present invention provides a new generation of DNA-PK inhibitors which are not only capable of specific inhibition, which arises, in particular, in the case of cellular assays.