Translation of "Primary antibody" in German

C represents a control labeling with silver enhancement and without primary antibody.

In the second step, a primary antibody is bound to the adsorbed antigen.

However, the primary antibody should bind the target protein specifically and with high affinity.

I do not know the concentration of my primary antibody.

The incubation time for the primary antibody was one hour at room temperature.

During the analysis, sample is added along with the primary antibody specific for the target toxin.

Is the ReadyTector® solution with my valuable primary antibody re-usable?

Can ReadyTector® be used with any primary antibody?

You only have to add your specific primary antibody.

You can save solution and primary antibody by re-using the solution with the antibodies up to 5 times.

The primary antibody incubation period can vary greatly depending upon which protocol a researcher is using.

The primary antibody can be omitted to determine the non-specific staining by the secondary antibody.

A sample processed without the primary antibody was used as a negative control.

More than one secondary antibody can attach to each primary antibody which increases the fluorescence signal.

In a third step there is addition of another, so-called secondary, antibody which has specific binding sites for the primary antibody which thus in turn acts as antigen.

For control in flow cytometry W6/32 mab against MHC class I was used as the primary antibody.

The blots were incubated for 1 hour each with of the primary HSP72 antibody (Amersham) and the secondary antibody (goat anti-rabbit IgG peroxidase conjugated, BioRad).

The concentration of the LDL receptor is measured by ELISA methodology, with the C7-LDL antibody being used as the primary antibody.