Übersetzung für "Flag tag" in Deutsch

Particular preference is given to affinity tags from the group consisting of His-tag, Flag-tag, Arg-tag, c-Myc-tag and Strep-tag.

A flag-TAG was inserted at the N-terminal end, in order to demonstrate that the FZD receptors are membrane-bound.

In the search operation for a specific entry in the buffer memory, this offers the advantage that both the banks of the data buffer and the tag/flag memories assigned to the banks in which the page addresses of the individual entries are contained can be directly and immediately selected with the class address, because the class address portion of a user address remains unchanged in the address translation.

Moreover, the class with the new virtual bank address bits is selected in the same bank and a valid entry existing here is defined as invalid in the duplicate tag/flag memory.

If, however, as already mentioned, the address translation memory is built up and organized analogous to the buffer memory, then this can be driven with the virtual page address at the same time as the tag/flag memories and the data buffer banks respectively driven with the real class address, so that all three aggregates simultaneously offer the selected contents at their outputs after termination of the access time.

The method as claimed in claim 6, wherein the binding partners A and B are chosen such that they form a binding pair A/B selected from the group consisting of FLAG-tag/anti-FLAG-tag antibody, His-tag/anti-His-tag antibody, fluorescein/anti-fluorescein antibody.

Particularly preferred binding pairs A/B may be FLAG-tag/anti-FLAG-tag antibody, His-tag/anti-His-tag antibody, and fluorescein/anti-fluorescein antibody.

As described in example 1, a thrombin-specific peptide substrate, comprising seven amino acid residues, which has a FLAG-tag epitope at the amino-terminal end and is biotinylated at the carboxy-terminal end was bound to the solid phase (microtiter plate) via an anti-FLAG antibody.

Subsequently, a thrombin-specific peptide substrate comprising seven amino acid residues, specifically leucine, valine, proline, arginine, glycine, phenylalanine, glycine in said sequence, which has a FLAG-tag epitope at the amino-terminal end, and which is biotinylated at the carboxy-terminal end was incubated with the solid-phase-bound anti-FLAG antibody and thus bound to the solid phase.

Especially preferred binding pairs A/B are FLAG-tag/anti-FLAG-tag antibodies, HIS-tag/anti-HIS-tag antibodies, fluorescein/anti-fluorescein antibodies, biotin/avidin and biotin/streptavidin.

The method as claimed in claim 6, wherein the binding partners A and B are selected to form a binding pair A/B selected from the group consisting of FLAG-tag/anti-FLAG-tag antibody, His-tag/anti-His-tag antibody, fluorescein/anti-fluorescein antibody.

The substrate ligands are usually synthetic, low molecular weight peptide substrates which have two artificial residues for association with the signaling components, for example an amino terminal Flag tag and a carboxy terminal biotin residue.

The reporter molecule can be chosen, e.g., from a group comprising His-tag, Flag-tag, a fluorescence label, biotin and streptavidin.

In this context, so-called tag sequences are preferred in the framework of the present invention, for example a flag tag, in other words the amino acid sequence DYKDDDDK, or also, for example, a His tag (containing several consecutive histidines).

Examples of the protein tags known to the person skilled in the art are a (His) 6 tag, a Myc tag, a FLAG tag, a haemagglutinin tag, glutathione transferase (GST) tag, intein having an affinity chitin-binding tag or maltose-binding protein (MBP) tag.

Examples of protein tags known to the skilled worker are an N- or C-terminal (HIS) 6 tag, a myc tag, a FLAG tag, a hemagglutinin tag, glutathione transferase (GST) tag, intein with chitin-binding affinity tag or maltose-binding protein (MBP) tag.