Übersetzung für "Loading buffer" in Deutsch

The column was washed with 7 ml loading buffer in order to wash out polyA RNA.

The His-Tag-7?-HSDH protein was eluted with elution buffer (200 mM imidazole in the loading buffer).

The sample was human serum, diluted 1:19 with loading buffer.

Then 1.0 ?g/ml monoclonal anti-human IgG-antibody-digoxigenin conjugate in loading buffer was added and it was washed.

In accordance with the desired test concentrations, the substances were pre-diluted in loading buffer (see above).

Loading Buffer IV is used for DNA samples dissolved in water or TE buffer.

Loading Buffer II is used for DNA samples dissolved in water or TE buffer.

Then 0.2 ?g/ml p24-digoxigenin in loading buffer was added, incubated for 20 min and it was washed.

Finally, the cells are admixed with 10 ?l of agonist solution (acetylcholine dissolved in loading buffer).

Run at least 12 µl of amplified DNA reaction mixture from each sample mixed with 3 µl loading buffer (Appendix 6) in 2,0 % (w/v) agarose gels in tris-acetate-EDTA (TAE) buffer (Appendix 6) at 5 to 8 V per cm.

The buffer loading the station track is preferably timed by a control line from the station track, which provides a measured supply of vehicles into a space present on the conveying cable.

The phenol-treated samples were lyophilized, taken up in 95% formamide loading buffer and fractionated by electrophoresis on a 20% polyacrylamide gel with 8 M urea at 55° C.

Then immediately 3 ?l are removed by pipette for the 0 value and added to 3 ?l of formamide loading buffer in order to stop the enzyme activity.

Total RNA resuspended in 500 ?l sterile H2 O was diluted with 4.5 ml loading buffer, heated for 5 min at 65° (water bath) and loaded on the prepared oligo(dT)-cellulose column.

For each cell line, 50 ?g of protein were diluted in SDS sample loading buffer (62.5 rnM Tris-HCl (pH 6.8), 2% SDS, 10% glycerol, 2% DTT, 0.001% bromophenol blue), which had been boiled for 5 min, separated on a 7.5% Tris-HCl gel (Biorad, Munich, Germany) and transferred on to a PVDF membrane (Millipore, Schwalbach, Germany).

What can be seen outside the overlapping area are outputs 10 of the buffering device 2 for loading the pouches 6, unloading stations 11 for additionally feeding out mail items 4 from the pouches 6 according to specific sorting criteria, a loading station 12 for loading the buffer pouches with the mail items from the singling device 1, and an output 13 of the buffering device 2 for feeding out separated mail items.

The figure shows outputs 10 of the buffer storage device 2 outside the coverage area for loading the pockets 6, unloading stations 11 for additional removal of postal items 4 from the pockets 6 according to specific sorting criteria, a loading station 12 for loading the buffer pockets with the postal items from the separation device 1 as well as an output 13 of the buffer storage device 2 for removal of separated postal items.

For each cell line, 50 ?g of protein were diluted in SDS sample loading buffer (62.5 mM Tris-HCl (pH 6.8), 2% SDS, 10% glycerol, 2% DTT, 0.001% bromophenol blue), which had been boiled for 5 min, separated on a 7.5% Tris-HCl gel (Biorad, Munich, Germany) and transferred on to a PVDF membrane (Millipore, Schwalbach, Germany).

The supernatant was loaded onto a Talon column (Clontech, USA) equilibrated with loading buffer (50 mM potassium phosphate, 300 mM NaCl, pH 8).

Weakly binding protein was removed by washing with washing buffer (20 mM imidazole in the loading buffer; 3 column volumes).

The process was carried out at 24° C. Non-bound material was washed out by washing of the column with loading buffer (3 column volumes).