Übersetzung für "Cell washing" in Deutsch

After removing the cell culture supernatant, washing is carried out once with PBS.

The dirty work happens in the washing cell (Foto: KUKA)

The last extraction cell 24m-1 can be followed by a so-called washing cell 24m, above which is arranged a spray head 36 which is connected to a washing-water line 37 via a valve.

After the washing cell 24m, the bottom 27 has a discharge cutout 29, so that, during the further transport of the cells, the cell content can fall downwards through this cutout 29 into a discharge and be conveyed away.

As is customary in microbiology, the cells are first split open through ultrasonic treatment, the cytoplasmic membrane fragments are disintegrated with the aid of detergents, and the remaining cell-membrane fragments are cleaned of the components contained in the cell by washing.

As is customary in microbiology, the cells are first split open through ultrasonic treatment, the cytoplasm membrane fragments are disintegrated with the aid of detergents, and the remaining cell-membrane fragments are cleaned of the substances contained in the cell by washing.

As is customary in microbiology, the cells are first split open through ultrasonic treatment, the cytoplasmic membrane fragments are disintegrated with the aid of of detergents, and the remaining cell-membrane fragments are cleaned of the components contained in the cell by washing.

Free warm water, guarded and insured parking, safe deposit box, refridgerated boxes, loading of cell-phones, washing machines, free caravan placing, free Wi-Fi.

Typical liquids which are moved by microplate washing devices for washing biological samples are, for example, media for cell cultures, washing buffers, reaction solutions, solvents for biological samples such as nucleic acids, proteins or whole cells, or liquid residues of such biological samples.

If the cells are cultured cells in suspension or cells in a bodily fluid, the method step I) preferably comprises pelleting the suspended cells, where appropriate after removing particular cell populations, for example by means of a cell sorter, washing the cell pellet thus obtained and optionally resuspending in a suitable suspension buffer.

In one well, the LRP antagonist, RAP, was added immediately to a concentration of 20 mg/ml at 2 and 4 hours after cell washing.

In two of the wells for each Factor VIII variant, the LRP-antagonist RAP was added immediately to a concentration of 20 mg/ml and 2 hours after cell washing.

Samples were drawn two and three hours after cell washing and then analyzed for Factor VIII cofactor activity using an established method (Mertens K. et al., Brit.

Since the equilibrium of intracellular NH 4 + with NH 3 and H + was disturbed by the removal of the extracellular NH 4 + and by the subsequent instantaneous passage of the NH 3 through the cell membrane, the washing process resulted in H + remaining inside the cells, which was the cause of the intracellular acidification.

In one well, LRP antagonist, RAP, was added immediately to a concentration of 20 mg/ml at 2 and 4 hours after cell washing.

Samples were drawn up to six hours after cell washing and then analyzed for Factor VIII-cofactor activity using an already established method (Mertens K. et al., Brit.

In three of the wells, the LRP-antagonist RAP was added at a concentration of 1 ?M at 2 and 4 hours after cell washing.

Samples were drawn up to 6 hours after cell washing and then analyzed for their Factor VIII A3-C1 concentrations.

A cleaner at the BMW plant in Dingolfing has to work under extremely harsh conditions: In the middle of a washing cell at 60°C.

Free warm water, insured parking, safe deposit box, refridgerated boxes, loading of cell-phones, washing machines, free caravan placing, free Wi-Fi.

In the case of immunocytological detection methods with intracellular staining, the following situation results with the detection method according to the invention: After the temporary generation of holes in the cell membrane, the introduction of the peptide-blocked fluorescence-stained antibody into the cell, the subsequent washing and closing of the pores, a certain proportion of the fluorescence labeled detection antibody remains in the cell (some unbound but not washed out and some non-specifically bound).

The spleen cells are washed one or more times and then fused with cells of a mouse myeloma line.

The cells are then washed with DMEM and suspended in the same nutrient medium.

The cells are subsequently washed a further two times with washing buffer.

The cells were then washed, resuspended in PBS and analyzed in the FACS.

The cells were subsequently washed twice with sterile NaCl solution (0.9%).

After harvesting, cells were washed twice with PBS and pelletized.

After growth the cells were washed off and plasmid DNA was prepared therefrom.

After removal of the culture medium, the cells are washed once with RPMI medium without serum.

The cells are washed with serum-free culture medium and centrifuged again at 400 g.

The cells were washed with ice-cold PBS and the dishes were out on ice.

The liposome suspension was then sucked off and the cells were washed with PBS.

Thereafter, the cells were washed again and incubated with culture medium for 24 hours.

Cells were washed and lysed by addition of 50 ?l of water.

The glass substrates with the cells were then washed three times with distilled water.

The cells were washed on day 10 and restimulated with fresh APC.

After incubation for one hour, the virus inoculum was drawn off and the infected cell layers were washed.

The cells were then washed and lysed in the well.

The cells were washed and lysed by addition of 50 ?l water.

The cells are then washed and lysed using ammonia solution.

After a one-hour incubation, the virus inoculum was removed and the infected cell lawn was washed.

The cells are then washed and lysed using methanol.

Subsequently the cells are washed twice with ice-cold cell culture medium.