Translation of "Oxalacetate" in German

Pyruvate is produced, for example, by decarboxylation of oxalacetate, the transamination product of aspartate.

This gives the L-amino acids corresponding to the a-keto acid and oxalacetate from the aspartic acid.

Aspartate is formed from oxalacetate, an intermediate of the citrate cycle.

Aspartate is formed from oxalacetate, an intermediate product of the citrate cycle.

Oxalacetate is used for the glucose biosynthesis and is unavailable for the reaction with acetyl-CoA.

A reagent suited for use with this method contains ?-aminobutyrate transaminase, succinate semialdehyde dehydrogenase, ?-aminobutyrate, glutamate and buffer, as well as either oxalacetate or pyruvate, and optionally a tetrazolium salt, an electron carrier and a surface-active agent.

Thus, for example, serum always contains varying amounts of acetoacetic acid which simulate the oxalacetate which is first to be formed in the GPT or GOT reaction.

However, it is also possible to couple the oxalacetate formed according to equation (2) with a diazonium salt to give a dye which can then be photometrically evaluated, such a procedure having been described by A.

The present invention also provides a reagent for the determination of GOT and GPT, which comprises ?-aminobutyrate transaminase (GAB-GT), succinate-semialdehyde dehydrogenase (SS-Al-DH), ?-aminobutyrate, glutamate and buffer, as well as either oxalacetate or pyruvate and optionally a tetrazolium salt, an electron carrier and a surface-active agent.

The ?-aminobutyrate, glutamate, oxalacetate and pyruvate are salts of the acids in question with cations which do not impair the enzymatic reaction.

An alternative possibility in a coupled process involving a phosphinothricin-specific transaminase and a GOT is for the reaction to be driven by the GOT in the direction of phosphinothricin synthesis by recycling the glutamate consumed by the phosphinothricin transaminase, because the GOT reaction product, oxalacetate, is spontaneously decarboxylated to pyruvate in the presence of doubly charged metal ions and is thus removed from the reaction equilibrium.

The increase in the yield of L-phosphinothricin by the decarboxylation reaction of oxalacetate under the stated reaction conditions was completely unexpected because, according to UK Patent Application 2 161 159, the highest product yield was found only with aspartate as sole amino donor in the reaction system.

The virtually complete consumption of the amino donors aspartate and glutamate results in the product L-phosphinothricin being virtually free of natural amino acids, and separation of it from the formed a-keto acids a-ketoglutarate and oxalacetate or pyruvate is possible in a single step.

Only then has sufficient pyruvate or oxalacetate formed to enable the hydrazone formation to be initiated and this requires a further incubation period.

Thus, for example, serum always contains varying amounts of acetoacetic acid which simulates oxalacetate which is first to be formed by the GOT reaction.

A conventional and much-used process for the determination of GPT/GOT employs the transamination of a-keto-glutaric acid with alanine and/or aspartate which is catalysed by this enzyme and leads to the formation of glutamate and pyruvate or oxalacetate.

Pyruvate or oxalacetate is reacted by the reduction with NADH in the presence of lactate dehydrogenase(LDH) and optionally of MDH into lactate or malate and NAD+, wherein the latter is measured according to the prior art methods either directly in UV or with the aid of a subsequent color reaction.