Translation of "Linearised" in German

Circular or linearised shuttle vector DNA can be employed for the cotransfection.

The thus determined position data are converted by a subsequent correction table into linearised position data.

The laser is linearised throughout the entire measurement area.

The task of the linear two-variable system is to decouple the system, which is linearised about a specific working point.

The linearised form of pRH77 is purified by agarose gel electrophoresis and elution of the DNA with subsequent precipitation.

Circular, double strand DNA molecules were linearised with a restriction endonuclease and used without further purification.

Subsequently, E1B55k in pShuttle was linearised with ApaI, the ends blunt ended and cut with NheI.

Subsequently, E1B55kDa in pShuttle was linearised with ApaI, the ends blunt ended and cut with NheI.

The resulting DNA fragment was ligated in the vector pZ1 previously linearised and dephosphorylated with ScaI.

To achieve the greatest possible precision, the sensor characteristic is linearised using an internal compensation algorithm.

For the transfection all plasmids except pGS122 were linearised prior to the transfection by a digest with suitable restriction nucleases.

The measurement supplied by the resistance sensor is linearised and converted to °C.

All values, which are transmitted by the weather station via 2 wires, are linearised.

As a result of the subtraction of the output signals of the two radiation receiving elements 29a and 29b, the characteristic line of the position sensor arrangement, i.e., of the complete arrangement of the pulse radiation barriers 8a and 8b and the position sensor operating circuit 9 is linearised in the overlapping working region which is defined above.

Process for preparing the plasmid parpATER103, characterised in that a 0.47 kb long fragment of the plasmid parpER33 which has been totally cut with HindIII and partially cut with EcoRI is inserted by ligase reaction into the plasmid PAT153 which has been linearised by restriction endonuclease digestion with EcoRI and HindIII, and the resulting plasmid is transformed for replication in E. coli HB 101 and cultivated.

For example, a vector, especially pBR322, is cleaved with a restriction endonuclease and, optionally after modification of the resulting linearised vector, an expression control sequence provided with corresponding restriction ends is introduced.

In order to prepare the expression plasmid pRH 100, the plasmid pER 103 (Eva Dworkin-Rastl et al., Gene 21 (1983) 237-248, EP-A-0.115-613) was linearised with the restriction endonuclease HindIII and the 5' terminal phosphate residues were removed.

In a preferred embodiment of the present invention, a vector derived from pBR322 that contains an expression control sequence, especially that of the tryptophan operon (trp po), which at the 3'-end (between the main mRNA origin and the first ATG) carries the recognition sequence for a restriction endonuclease preferably forming cohesive ends, for example EcoRI, is digested with the mentioned restriction endonuclease and, in the vector DNA fragment, with a second restriction endonuclease that forms blunt, or preferably cohesive, ends, for example BamHI, after which the so linearised vector is linked with the gene having corresponding ends (for example with an EcoRI end before the ATG start and a BamHL end after the translation stop codon) that codes for one of the amino acid sequences according to the invention.

The manufacture of the linearised vector obtained is also described in "Hans Rink et al., Nucleic Acids Research 12, 6369-6387 (1984)" in which the plasmid referred to in the present Application as pHRi148 is called pHR 148.