Übersetzung für "Sugar phosphate" in Deutsch

The sugar phosphate used in excess can be retrieved by regeneration of the reaction solution.

Internal modifications can affect the bases, the sugar-phosphate backbone or inserted side chains.

Internal modifications can affect both the bases and the sugar-phosphate backbone.

Internal modifications can affect the bases or the sugar-phosphate backbone.

Internal modifications can involve the bases or the sugar-phosphate backbone.

A nucleotide contains a nitrogenous base, a ribose sugar, and a phosphate.

The bases are connected with each other by their sugar-phosphate backbone.

Subsequently, the sugar phosphate is added to the resin and shaken for 6 d at RT.

The “feed” RNA can carry several modifications both to the bases and in the sugar-phosphate backbone.

For this the preferred solution is the attachment of at least one phosphothioate instead of a phosphate in the sugar-phosphate backbone of the DNA oligonucleotide.

The preferred solution for this is the incorporation of at least one phosphothioate in the sugar-phosphate backbone of the DNA oligonucleotide in place of a phosphate.

The nucleotides are joined to one another in a chain by covalent bonds between the sugar of one nucleotide and the phosphate of the next, resulting in an alternating sugar-phosphate backbone.

It is also contemplated that appropriately modified chromophores of the lumazine type may be bonded to appropriately modified bases or to the sugar-phosphate backbone.

The conjugate of macromolecule and second partner of the binding pair used within the scope of the invention can preferably be a conjugate which contains a protein as the macromolecule that can, if desired, be modified by sugar, phosphate or/and lipid residues, e.g. an unspecific antibody i.e. an antibody (MW ca. 150000) which does not exhibit specific binding to any component of the test system or another protein such as albumins (MW 68000) like bovine serum albumin or aggregated albumin (MW>1 million, cf. EP-A 0 269 092).

The following are of primary suitability: calcium carbonate, pulp meal (ground wood meal, cotton or the like), carbohydrate (starch and/or sugar), dicalcium phosphate, very finely ground meat meal or bone meal, fish meal, milk protein, soybean flour, fat, fatty acid, polyvinyl alcohol, talcum, polyethylene, polypropylene, polyamide and/or polyester.

Thus, for example, binders, such as, for example, gelatin, sorbitol, polyvinylpyrrolidone or carboxymethylcellulose, diluents, such as, for example, lactose, sugar, starch, calcium phosphate or polyethylene glycol and lubricants, such as, for example, talc or magnesium stearate are suitable for capsules or tablets, and, for example, aqueous or oily suspensions, syrups or similar known preparation forms are suitable for liquid preparations.

Stabilization against nucleolytic degradation can be effected by modifying or replacing the phosphate bridge, the sugar unit, the nucleic base, or by replacing the sugar-phosphate backbone of the oligonucleotides.

The synthesis of defined sequences is achieved if, for example, a polyamide backbone, which is built up stepwise in analogy with conventional peptide synthesis (M. Bodanszky, Principles of Peptide Synthesis, Springer, Berlin, 1984), is used in place of sugar phosphate.

Those which may be mentioned by way of example are the peptide nucleic acids (PNA's), in which the sugar/phosphate backbone is replaced by an aminoethylglycine backbone (see German Patent Application P4408531.1), and the carbamate-bridged morpholino oligomers.

Stabilization against nucleolytic degradation can be achieved by replacing the sugar-phosphate backbone or by modifying the phosphate bridge, the sugar moiety or the nucleotide base Milligan et al., above and Uhlmann & Peyman, above!.