Übersetzung für "Gradient elution" in Deutsch

In liquid chromatography this is called gradient elution.

The following buffers are used for the gradient elution of the proteins:

However, it is also possible to use an elution gradient.

Active fractions were obtained in the elution gradient between 400 to 0 mM ammonium sulfate.

The HPLC run was carried out with gradient elution.

Separation is performed based on hydrophobic interaction and gradient elution.

Finally, this is followed by elution with eluents, optionally with gradient elution, at a certain flow rate and by detection.

With this packing the separation of homologeous fatty acids by gradient elution is no problem (Application FS5).

This is followed by an isocratic RP separation using 25% water and 75% methanol as elution gradient.

The elution of the mixture of nikkomicin (X) and nikkomicin (Z) can be carried out by the generally customary methods, for example by simple stirring with solutions of increasing acid concentration (preferably using acetic acid), or by gradient elution over a column.

The adsorbents described therein are suitable for column chromatography, in particular for high-pressure liquid chromatography or gradient elution.

The water/alcohol mixture used as an eluant in the chromatography may be used in isocratic form, i.e. with a constant solvent ratio, or with a continuously changing solvent ratio (gradient elution).

Although cationic or anionic gels based on polysaccharides bind the dextran-rich phase as well as cellulose does, they can only be used for isocratic separation processes, since the phase is repelled as soon as the electric phase potential changes in the course of the gradient elution with various salts in the mobile, polyethylene-rich phase.

The ion exchange can be effected with the aid of gradient elution, for which both pure pH gradients, pure salt gradients and mixed pH/salt gradients can be employed successfully.

The organic phase was dried over MgSO4, concentrated and the residue was chromatographed on silica gel with diethyl ether/hexane 1:5 to 2:1 (gradient elution).

The adjustment of the pH gradient and the elution of the protein were effected by application of 50 ml polybuffer PB74 (Pharmacia, Freiburg, Germany) pH=4.0 to the column.

The solvent is removed by evaporation and the crude product is purified over a short column of 400 g of silica gel (gradient elution with hexane/toluene).

The crude product is purified over a short column of 400 g of silica gel (gradient elution with hexane/toluene/2% ethyl acetate).

Surprisingly, it has been found that it is possible to bind SLO, for example from a culture solution, in the presence of a water-soluble thiol compound and a sufficiently high concentration of ammonium sulfate to phenyl-RTMSepharose or another support suitable for hydrophobic interaction chromatography, in a buffer, and to separate it from other protein components by gradient elution in a decreasing ammonium sulfate gradient.

The invention therefore relates to a process for the purification of streptolysin O (SLO), wherein a SLO-containing solution is brought into contact, in the presence of a water-soluble thiol compound and a salt, with a material suitable for hydrophobic interaction chromatography, in a buffer, and eluted by gradient elution with a solution of a salt using a negative concentration gradient, which starts at a concentration which is lower than the concentration of the salt for the adsorption, and the fraction which contains the SLO is obtained.