Übersetzung für "Enzymatic digestion" in Deutsch

In this method the tissue sample is subjected to an enzymatic digestion.

The enzymatic digestion is performed by means of pancreatic lipase (EC 3.1.1.3).

The resulting lysobactin fragments dihydrolysobactin and octahydrolysobactin are then used for the enzymatic digestion.

The tissue particles are prepared by the mechanical comminution of the tissue material removed and, if necessary, by a following enzymatic digestion.

Enzymatic DNA digestion has been designed in such a way that it can be conveniently run during the RNA isolation process.

Soluble oat flour is prepared by enzymatic digestion of oat flour (e.g., by alpha amylase).

In this, the mash production and the enzymatic digestion of mash for fermentation was supposed to be optimised.

The enzymatic digestion of uric acid to allantoin by rasburicase produces hydrogen peroxide and haemolytic anaemia or methaemoglobinaemia have been observed in certain at risk populations such as those with G6PD deficiency.

Therefore a HPLC method including an enzymatic digestion of the vitamin E formulation without an alkaline saponification step is to be recommended for confirmation.

This derives from new demands, advances in technology (e.g. enzymatic digestion of cellulosic material, engineering of food properties, crop breeding including genetically modified organisms etc.) and changes in farming practices.

The enzymatic or acidolytic digestion of dietary proteins leads to a nearly complete loss of the antigenicity, but the oligopeptide or elemental diets produced by this technique result in a drastic deterioration in taste, which causes almost generally a refusal of those foods by the patients.

These fragments are preferably peptides which can be synthesized by enzymatic digestion of the intact antibodies or by chemical reactions according to methods known to the expert.

However, care has to be taken that the necessary cell density is achieved in the cell space possibly by partly separating the cells from their extracellular matrix, e.g. by means of a short enzymatic digestion.

Subsequently, the enzymatic digestion of the embryonic tissue was effected by the addition of proteolytic enzymes (1 mg/embryo) at 37° C. for 20 minutes.

The isolated protein domains according to claim 15, said protein domains comprising: domain gp120 of the HIV env-glycoprotein and the aminoterminal portion of domain gp41 of the HIV env-glycoprotein said aminoterminal portion further comprising an aminoterminal amino acid sequence having a length in the range of from 131 to 165 amino acids following said enzymatic digestion.

According to the invention, enzymatic digestion is preceded by a cell-protecting, mechanical comminution in such a way that tissue particles are obtained, which are as far as possible of the same size.

Sedimentation can be accelerated, e.g., by an appropriate choice of the depth of the sedimentation vessel, by diluting the suspension at the end of enzymatic digestion and/or by other suitable measures.

The different steps required for this, e.g. the mechanical cutting of the patient's own starting tissue and also the subsequent enzymatic digestion, are performed in the form of a closed process in a machine, which is not described here.

The byproducts resulting from the enzymatic digestion need not necessarily be removed from the suspension before the carrier is treated therewith.

Surprisingly, the canine model has also revealed that there is no need to separate the fatty phase following enzymatic tissue digestion.

According to the invention, tissue particles with a very uniform size and a large number of vital cells can be obtained in that the mechanical comminution stage and the enzymatic digestion stage are precisely matched to one another.

Sedimentation and separation of the resulting layers is preferably performed immediately following enzymatic digestion, preferably in the same vessel and at 20° to 40° C., preferably 30° to 37° C., for adipose tissue.

The first process step consists of the isolation of the microvascular cells desired for incorporation as a result of the mechanical comminution of the tissue removed from the patient, followed by enzymatic digestion of the comminuted tissue with a suitable enzyme and the separation of all undesired constituents from the suspension prepared by digestion for the isolation and concentration of the microvascular cells.

A particularly promising feature in this respect are the in vitro enzymatic digestion testa which in many cases can give biologically correct and valid results within 24 hours or leBs.

A particularly premising feature in this respect are the in vitro enzymatic digestion teBts which in many cases can give biologically correct and valid results within 24 hours or less.