Übersetzung für "Electroporated" in Deutsch

This ligation mixture was then electroporated into strain Corynebacterium glutamicum ATCC13032.

Mature DCs were electroporated with increasing RNA concentrations.

A Cre expression vector is simultaneously electroporated into the cells;

At the same time, the Flp expression vector pOG44 is electroporated into the cells (conditions for this see Example 1d).

At the same time, the Cre expression vector pMC-Cre is electroporated into the cells (condition for this see Example 1d).

In order to modify the ES cells, R1 cells from passage 16 were electroporated with various constructs;

Immediately after electroporation, half of the immaturely electroporated cells were matured (iDCm).

After electroporation, half of the immaturely electroporated cells were treated with maturation cocktail (iDCm).

As control conditions, DCs were electroporated without RNA or with irrelevant RNA (control RNA).

The mean fluorescence intensity (MFI) of the electroporated dendritic cells was determined by flow cytometry.

The highly complex mixtures of human vlambda vector DNA described in Example 5e (pBS FRTclone-vlambda) are electroporated into the cells of the expanded clone HEA125-mhloxPmycFRTG418 together with the Flp expression vector pOG44 (see Example 4c), the cells are stained with FITC-labeled goat anti-human kappa antibody after 3 days, and cells are isolated by means of a FACS sorter as described.

The vector pBS loxP-FdHEA described in Example 15a is electroporated into the HEA125-mhRek cell line described in Example 4d together with the Cre expression vector pMC-Cre as described in Example 1d and subsequently about 2,000 clones are propagated separately by limited dilution of the employed about 10 7 cells.

This group of selected hybridoma cells is expanded in a cell culture and then a mixture of the above described expression vectors is electroporated into these cells.

As described in Example 5f for vlambda, the highly complex mixtures, described in Example 7e, of human vH vector DNA (pBS loxPclone-vH) are electroporated into the cells together with the Cre expression vector pMC-Cre.

The vector pBS loxP-IgGdeltaCH1 (FIG. 16) is electroporated into the cells of the expanded Jurkat G418 cell line described in Example 18a together with the Flp expression vector pOG44, as described in Example 1d, and then 500 clones are propagated separately by limited dilution of the employed about 10 7 cells.

The highly complex mixture, described in Example 19b, of vector DNA is electroporated into the cells of the expanded clone Jurkat loxP-IgG1deltaCH1 together with the Cre expression vector pMC-Cre, the cells are stained using FITC-labeled goat anti-human IgG antibody after 3 days and cells are isolated by means of a FACS sorter as described.

First, R1 cells were electroporated with the c-fos expression vector and two G418-resistant clones designated R-169.2.3 and R-169.2.5 were used for injection into tetraploid blastocysts (the R1 clones were cultivated over more than 24 passages before being injected into blastocysts).

In cases where DNA or RNA are injected or electroporated into plant cells, the plasmid used need not meet any particular requirements.

The technical problem of the present invention is also solved by an installation for the extraction and isolation of constituents from sugar beet chips, comprising at least one device for comminuting sugar beets into sugar beet chips, at least one adjoining device for transporting the chip-juice mixture obtained, at least one adjoining device for transporting the chip-juice mixture obtained, at least one adjoining device for the electroporation of the chip-juice mixture, at least one adjoining one device for transferring the electroporated chip-juice mixture and at least one adjoining device for extracting the chip-juice mixture.

Retinal explants from stage (st) 20 (embryonic days [E] 3) to st31 (E7) chicken embryos were dissected, cultured and electroporated with a defined DNA plasmid concentration or exposed to a medium containing a defined concentration of a chemical reagent.

The invention also provides that the comminuted plant materials, in particular beet cossettes, are introduced, in particular mingled, into a transporting liquid, in particular a beet extract or water, and then washed into an electrical reaction chamber, preferably formed as a tube or shaft reactor, preferably an electroporation device, then compacted into a plant material pack, in particular cossette pack, of a specific packing density and, in the form of this packing density obtained, conveyed through the electrical reaction chamber, in particular through the electroporation shaft or tube reactor, and electrically treated, in particular electroporated, it being advantageously possible for the speed of the comminuted plant material, in particular that of the beet cossettes, as well as the density of the cossette pack, to be specifically set by the sucking element and the conveying element.

The conveying element serves the purpose of conveying the electrically treated, in particular electroporated, materials, in particular cossettes, at a defined speed, while the sucking element, preferably arranged downstream of the conveying element, sucks in the mixture of comminuted plant material and transporting liquid, in particular the beet-cossette/transporting-liquid mixture, which is advantageously also accompanied by the effect that water is sucked through the cossettes.

In a further preferred embodiment, it may be provided that, in the installation according to the invention, the device for discharging the electrically treated plant material, that is to say the mixture of comminuted plant material and transporting liquid, in particular electroporated beet-cossette/transporting-liquid mixture, is arranged perpendicularly or substantially perpendicularly in relation to the tubular or shaft-like transporting device.