Übersetzung für "Dna duplex" in Deutsch

Duplex DNA molecules may, but need not be, subjected to an amplification reaction.

This enzyme will also repair single stranded nicks in duplex DNA, RNA or DNA/RNA duplexes.

The enzyme attacks the 3' hydroxyl at duplex DNA with blunt ends.

Using a suitable primer, a single-stranded or else duplex DNA is provided, prepared, obtained or otherwise acquired for the subsequent steps in a reverse transcription reaction.

In a subsequent step, duplex DNA is prepared, obtained or otherwise acquired from any existing single-stranded DNA with the aid of a suitable primer.

The term “plasmid”, as used herein, generally relates to a construct of extrachromosomal genetic material, usually a circular DNA duplex, which can replicate independently of chromosomal DNA.

The synthesis of the DNA sequence of formula IV can accordingly be carried out, for example, by building up two oligodeoxynucleotides, namely a negative and a positive strand having approximately the same number of bases, these oligodeoxynucleotides having the capability in a partial region of forming hydrogen bonds with each other, by means of condensing trinucleotide blocks, in stages, onto a growing nucleotide sequence according to the solid phase process, by detaching the individual nucleotide chains from the solid carrier, removing any protecting groups present, carrying out purification, for example by means of gel-electrophoresis, and, after kinasing and hybridising, making up to the complete DNA duplex, preferably by means of DNA polymerase I (Klenow fragment).

The DNA bound to the surface is treated, preferably with a bisulfite (=disulfite, hydrogen sulfite), in such a way that cytosine is converted into a base that is different in its base pairing behavior in the DNA duplex, while 5-methylcytosine remains unchanged.

It is also preferred that a reagent denaturing the DNA duplex and/or a radical trap is present in the case of the chemical treatment.

It is also and additionally preferred that in the chemical treatment, a reagent that denatures the DNA duplex and/or a radical trap are present.

Then the DNA bound to the surface is treated, preferably with a bisulfite, in such a way that cytosine is converted into a base that is different in its base pairing behavior in the DNA duplex, while 5-methylcytosine remains unchanged.

In another particularly preferred method variant, in the chemical treatment, a reagent that denatures the DNA duplex and/or a radical trap is present.

The method according to claim 4, further characterized in that a reagent denaturing the DNA duplex and/or a radical trap is present in the chemical treatment.

The method according to claim 4, further characterized in that in the chemical treatment, a reagent that denatures the DNA duplex and/or a radical trap is present.

It is also and additionally preferred that a reagent that denatures the DNA duplex and/or a radical trap is present in the chemical treatment.

This enzyme can link blunt end and cohesive end termini, repair single stranded nicks in duplex DNA and RNA or DNA /RNA hybrids.

The duplex DNAs formed during complementing have blunt ends.

The duplex DNAs formed during complementing, which are especially fragments of the desulphatohirudin gene (process e1) or are the complete desulphatohirudin gene (process e2), have blunt ends.

The duplex DNAs formed during complementing, which are especially fragments of the desulphatohirudin gene (process el) or are the complete desulphatohirudin gene (process e2), have blunt ends.

Untie the knot DHX9 has the ability to unwind DNA and RNA duplexes and plays a central role in many processes in the cell like DNA replication, transcription or RNA processing.