Translation of "Restriktionsverdau" in English

Correspondingly positive clones are identified by analytical restriction digest and sequencing.

The correct orientation of the bioS1 fragment in pHS1 was determined by restriction digestion and sequencing.

Correspondingly positive clones are identified by analytical restriction digest and sequencing.

Correct orientation of the bioS2 fragment in pHS2 was determined by restriction digestion and sequencing.

Two DNA fragments were generated by the restriction digest.

Positive clones were identified and sequenced after a restriction digestion with the enzyme Xho1.

The completeness of all plasmids described above was tested by restriction digest.

The plasmid was prepared starting with a Bam HI and Hind III restriction digest.

Positive clones were identified after a restriction digestion with the enzyme EcoRI and several clones were sequenced.

Once the single colonies had appeared, they were tested for correctness by isolating their plasmids and subjecting them to restriction digestion.

The DNA fragments which were obtained in Example 1 by means of PCR and subsequent restriction digestion were ligated into this linker.

This was carried out by restriction digestion with Bam HI and Sal I and ligation into the corresponding pYT vector fragment.

Labeled oligonucleotides have found an extremely large number of uses in genetic engineering because they are easier to handle than the DNA probes which are conventionally used as hybridization probes and are prepared from native gene material by restriction digestion.

The position of the restriction sites within the lambda insert was determined using a method described by Rackwitz et al. (4) after partial restriction digestion of the lambda DNA, labelling of the right or left sticky ends of the lambda arms with synthetic P-32-labelled oligonucleotides and electrophoresis in 0.45% agarose gels.

The restriction digestion of the isolated DNA, the gel-electrophoretic separation of the DNA with Agarose and the transfer of the DNA to a nylon membrane is described in Maniatis et al. (1982).

The plasmid DNA was isolated from the positive E. coli clone and a gene map was created by digestion with various restriction enzymes.

The plasmid was called pLeu1 (see plasmid map, FIG. 1a). Plasmid pLeu1 was used to prepare plasmid pLeu2 (FIG. 1b), in which the non-translated region at the 5' end of the gene was shortened to 400 bp by digestion with the restriction enzyme SmaI.

After separation of the smaller restriction fragments, the construct is ligated to short hairpin-formed self-hybridizing oligodesoxynucleotides, said hairpin-formed desoxynucleotides providing a overhang able to hybridize to the overhang resulting from the restriction enzyme digestion of the construct (FIG.

The plasmid DNA (pMOS-FDH-C23S) was isolated from the recombinant pMOS blue cells after multiplication in E. coli and the FDH-C23S-EcoRl/Pstl fragment (1.1 kb) was prepared by means of restriction digestion.

It was an object of the invention to provide a simple method for the specific detection of variant nucleic acids which does not require digestion by restriction enzymes and allows amplifying the nucleic acids in one reagent mixture.

Positive clones were identified after a restriction digestion with the enzymes Xho1 and BamHI and several clones were sequenced.

Subsequent restriction digestion with the enzymes AsnI (Boehringer Mannheim) and PacI (New England Biolabs) yielded the desired DNA fragment after the enzymes had been removed.

Experience has shown that, as compared to cells with the capability of unlimited proliferation, nuclear chromosomal DNA of normal transient proliferating cells has, after restriction digestion and analysis of the restriction fragments in a southern blot, another pattern of hybridizing restriction fragments when hybridizing a DNA sequence in accordance with the invention.

An EcoRI fragment 1761 bp long and containing the second half of the panBC cluster was now cut out of the plasmid pUR1 by means of restriction digestion.

For the antibody 25.2m/2H10, the isolated bands were then subjected to a restriction digestion using the enzymes Xhol and Spel (heavy chains) or Sacl and Xbal (light chains). The fragments obtained were cloned into the plasmid vector Bluescript KS (Stratagene) after the vector had first been cleaved with the restriction enzymes Xhol and Spel or Sacl and Xbal.

The heavy chain Fd fragment and the light chain were then subjected to a restriction digest using the enzymes Xhol and Spel (heavy chain) and Sacl and Xbal (light chain). After the plasmid vector pBSIIIHisEx (Connex) had been split by means of the restriction enzymes Xho I and Spe I and/or Sac I and Xba I, the obtained fragments were cloned into this vector and sequenced again.

Following restriction digestion with the appropriate restriction endonuclease, the cDNA fragment is isolated from an agarose gel and ligated to a vector which has previously been cut in an appropriate manner.