Translation of "Mediumwechsel" in English

The first medium change takes place after two days and then every three days.

The first medium change takes place after two days, then every three days.

After the poisons had been washed out by the usual medium change these areas were again overgrown.

The medium was changed every 48 hours.

Instead, the medium can be changed simply by suction or even by automatic rotation of the substrate.

The fraction of the non-rosetting cells (HPBL(B)) were cultured at a density of 5×107 cells in RPMI 1640+10% autologous plasma (30 minutes/56° C.heat-inactivated) with about 10 ?g/ml HBsi in a carbon dioxide incubator (medium change every 12 hours).

Medium: Complete medium contains HAM's F12 (Biochrom KG, Berlin) and 10% fetal calf serum (Boehringer Mannheim); the test substance is dissolved in medium, added at a concentration of 10-5 M and added freshly at each medium change.

Subsequently, this is transferred into a cell culture flask and cultured at 37° C. for 5–9 days, leaving the medium unchanged.

On the next day the non-adherent cells were removed by a change of medium (DMEM/5% FCS and 0.02 ml FGF from bovine retinae/ml medium).

Owing to its design and structure, the inventive apparatus is suitable for the culture of high cell concentrations with frequent and simple exchange of the medium whilst it enables the co-culture of high numbers of cells of two species under definitely identical culture conditions in the vertical orientation.

Another advantage resides in the fact that due to the use of the apparatus the cluttering of a high cell concentration with frequent and simple exchange of the medium becomes possible.

After the incubation time of 4 hours, the medium was changed, replacing the complex-containing solution with fresh cell culture medium.

Only a very small portion of the stem cells (approximately 3%) undergo spontaneous differentiation and are separated with the old medium with no problem when changing the medium.

Moreover, there is less risk that residual amounts of vitamin D-binding proteins remain in the system, that renature upon medium change and distort the determination.

This is particularly advantageous because important growth hormones excreted by the cells during cell growth are again made available to the cell culture system and are not diluted out by an exchange of medium.

After cell counting, stationary culturing and amplification of the chondrocytes for 14-21 days (2 times per week change of medium with serum-containing medium).

Assay The medium was changed after incubation at 37° C., 5% CO 2 and 95% humidity for 48 h.

The left half of the micrograph originally showed growing mamma carcinoma which were destroyed by the peptide/enzyme combination and purged by the second medium change after 48 hours.

After an incubation time of 24 hours, the medium is changed with renewed administration of substance, and another 24 hours of incubation.

After adaptation for 5 days, interleukin-1 is added to the medium, 8 units/well for the cell culture, 20 units/well for the tissue culture, and, over the treatment period of 8 days, likewise added again every second day on changing the medium.

After 10 days selection (medium changed every 2 days) the positive clones are isolated and expanded.

The cells are incubated at 37° C. under 5% CO 2, and the medium is changed on the 2nd or 3rd day after preparation.

This was followed by a change of medium (10-15 ml medium in a 75 cm 2 bottle, 20 ml in a 175 cm 2 bottle).

The cells are incubated at 37° C. and 5% CO 2 and the medium is changed on the 2nd or 3rd day after preparation.