Translation of "Immunoblotting" in German

The immunoblotting was carried out according to Blake et al., Anal.

This method has impressive separation capabilities and can easily interface with immunoblotting techniques.

The immunoblotting is effected directly thereafter, as set forth in Example 32.

Immunoblotting is considered the most general version of western blotting and is used in combination with chromatographic methods.

IgE-binding assays (such as Radio or Enzyme Allergosorbent Assay (RAST or EAST), Enzyme Linked Immunosorbent Assay (ELISA) and electrophoresis followed by immunoblotting with specific IgE-containing sera) are adequate methods.

For this reason the resulting plaques were again rinsed off the plates with complete medium and subsequently plated out from the phage suspensions obtained in this way, which had undergone renewed amplification, and the immunoblotting was carried out on minimal agar plates.

Use of complete medium plates for the immunoblotting always lead to very high non-specific reactivity of the filters with the sera used.

It emerged during the work that replica filters of these phage platings were unsuitable for immunoblotting, probably because of contaminants in the eluate.

A sample of the concentrated eluate was subjected to electrophoretic fractionation on 10% SDS-PAGE and examined for purity by means of staining with Coomassie brillant blue and immunoblotting.

The proteolysis products were analyzed by means of SDS-PAGE (sodium laurylsulfate polyacrylamide gel electrophoresis) and subsequent immunoblotting using a polyclonal rabbit serum, directed against gp120, which recognizes both gp120 as well as gp160.

Then, a total of ten fractions were removed and tested by SDS-PAGE and immunoblotting for their content of env proteins.

By immunoblotting it could be shown that the small 12 kd part of the helper, which is present between the internal cleavage sites (b) and (c), is indeed released into the extracellular surroundings together with the IgA-protease.

Content Introduction into the methodology used for molecular analysis of gene expression in mammalian cells: cultivation of mammalian cells, analysis of induced gene expression by RT-PCR and immunoblotting, detection of cellular stress with the aid of a reporter construct (GFP) and fluorescence microscopy.

Western blotting, also called immunoblotting, is a widely used technique to monitor protein expression in a cell or tissue extract based upon antibody binding to a specific protein of interest.

Other methods (e.g. counterimmunoelectrophoresis, immunoprecipitation; immunoblotting) can be used but are not necessarily useful for routine.

Other techniques (immunodiffusion, immunoblotting, RNA immunoprecipitation, etc.) are possible but not necessarily useful for routine.

The antibodies can be examined advantageously a) by the technique of direct or indirect immunofluorescence, b) by the immunoblotting technique, including by use of blot membrane-based microarrays, or c) by the luminescence technique.

In the immunoblotting technique, proteins or other antigens are immobilized on nitrocellulose membranes, nylon membranes or other membranes, which are then incubated successively with patient samples or other samples, enzyme-labelled antibodies and a colour reagent.

Linked to common techniques, such as, for example, mass spectrometry (MS), 2D gel electrophoresis (2DE), amino acid sequencing or immunoblotting, comprehensive analysis of the proteins present in the cell and the subcellular distribution thereof can be employed in order to identify novel proteins or protein functions and to indicate their involvement in certain cellular signal pathways.

Culture supernatants and cell extracts from all the samples were investigated for expression of the S-layer protein by SDS-PAGE and Western immunoblotting.