Übersetzung für "Protein moiety" in Deutsch

The average molecular mass is approximately 60,000 of which the protein moiety constitutes approximately 20,000.

The protein moiety of the molecule contributes about 58% of total molecular weight and consists of 165 amino acids.

The average molecular mass is approximately 60 kDa of which the protein moiety plus the carbohydrate part constitutes approximately 30 kDa.

The average molecular mass is approximately 31,300 daltons of which the protein moiety constitutes approximately 19,300.

The average molecular weight of Refixia is approximately 98 kDa and the molecular weight of the protein moiety alone is 56 kDa.

Plasmids containing synthetic DNA sequences are described which are suitable for the expression of the intermediate protein of the recombinant scu-PA (i.e., the unglycosylated protein moiety of the single chain prourokinase) in Enterobacteriaceae, especially in E. coli, with expression rates far higher than those obtainable according to prior methods.

In a further embodiment of a competitive test, antibodies to the protein moiety of a polyhapten are fixed to the porous material.

The enzyme is not glycosylated and also has no prosthetic group which would lead to inactivation during removal of the protein moiety.

The conjugation technique according to the invention is distinguished by the fact that the actual coupling between the labelling enzyme and the immunologically active ligand does not take place via the protein moiety of the labelling enzyme but instead via the oxidized carbohydrate moieties and subsequently introduced amino groups of the labelling enzyme.

Several papers describe the production of the unglycosylated protein moiety of scu-PA by recombinant techniques, in Escherichia coli (hereinafter E. coli), which is designated as "recombinant scu-PA" (recombinant scu-PA; proposed INN "Saruplase").

The protein moiety of actual interest can then be liberated from this by specific proteolysis or, if an acid-labile cleavage site has been introduced beforehand, by acid hydrolysis.

The invention is based on the surprising observation that it is not necessary, as had previously been assumed, to link an active compound to a particular carrier under defined conditions and then to administer the product but that, on the contrary, it is possible to employ therapeutically and/or diagnostically effective substances, which consist of a pharmacological active compound and at least one protein-binding molecular moiety, which are linked to each other by way of a spacer, directly as injectable medicaments since these medic-aments, after having been brought into contact with the body, bind covalently, by way of the protein-binding molecule, to body fluid or tissue constituents, predominantly to serum proteins, such that a transport form of the active compound is created in vivo, which transport form reaches the cells or the tissue which is/are the target of the active compound.

The invention is based on the surprising observation that it is not necessary, as had previously been assumed, to link an active compound to a particular carrier under defined conditions and then to administer the product but that, on the contrary, it is possible to employ therapeutically and/or diagnostically effective substances, which consist of a pharmacological active compound and at least one protein-binding molecular moiety, which are linked to each other by way of a spacer, directly as injectable medicaments since these medicaments, after having been brought into contact with the body, bind covalently, by way of the protein-binding molecule, to body fluid or tissue constituents, predominantly to serum proteins, such that a transport form of the active compound is created in vivo, which transport form reaches the cells or the tissue which is/are the target of the active compound.

The enzyme is not glycosylated and also has no prosthetic group which would lead to inactivation on removal of the protein moiety.

The additional inserted protein moiety not only in many cases stabilizes the protein, which is encoded by the cloned-in foreign gene, against breakdown by cellular proteases; it can also be used for detecting and isolating the hybrid protein which is formed.

It consists of a protein moiety (bacterioopsin) and a chromophore (retinal), and it occurs in the cell membrane of halobacteria in the form of the so-called purple membrane (hereinafter also called PM).

In addition, it must be accepted that the polyether residues, which act as spacers and linking element between protein and silicone moieties, because of their polymer distribution and the high molecular weight character associated therewith, do not leave the properties of the product unaffected and have the property profile of hybrid silicone-polyether protein copolymers rather than act as pure silicone-protein copolymers.

Although the polyether phosphate unit makes the polymers soluble in water, they also have a very hydrolysis-sensitive phosphoric ester function, meaning that the silicone and protein moieties can again be readily cleaved from one another.

The actual coupling reaction in this case is effected using a heterobifunctional conjugation method which acts on the protein moieties of the labelling enzyme and the immunological ligand.

This object is fulfilled according to the invention using a method whereby the viruses or the viral particles are separated from the protein moieties and/or nucleotides in the sample matrix using capillary electrophoresis and at the same time the electropherogram associated therewith is recorded and the fractions assigned to the analytes are identified as virus peaks in the electropherogram by spectroscopic interpretation with the aid of characteristic maxima.